| Factor |
Value |
| Molecular weight (ave.) of DNA base pair |
649 Da |
| Molecular weight (ave.) of amino acid |
110 Da |
| 1 g/ml DNA |
3.08 µM phosphate |
| 1 g/ml of 1 kb DNA |
3.08 nM 5’ ends |
| 1 mol pBR322 (4363 bp) |
2.83 g |
| 1 pmol linear pBR322 5’ ends |
1.4 g |
| 1 A260 double-stranded DNA |
50 µg/ml |
| 1 A260 single-stranded DNA |
37 µg/ml |
|
1 kb DNA: 333 amino acids of coding capacity = 36,000 Da
6.5 x 105 Da of double-stranded DNA (sodium salt)
3.3 x 105 Da of single-stranded DNA (sodium salt)
3.4 x 105 Da of single-stranded RNA (sodium salt)
|
| 10 kDa protein = 91 amino acids = 273 nucleotides |
